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A simple technique for the extraction of citrate from bile and urine has been developed using a nitrocellulose membrane. This was achieved by the use of a new device, consisting of a pre-filter and an extraction cartridge containing a nitrocellulose membrane. The analytical method is based on the colorimetric determination of ascorbic acid at 540 nm in the presence of nitric acid after a single extraction with 0.5 M HCl containing 0.1 M potassium nitrate. The method was validated and applied to the determination of citrate levels in bile and urine of rabbits. A correlation coefficient of 0.999 was obtained for the citrate-ascorbic acid system. The procedure was simple and rapid.The present invention relates to a method for stabilizing a semiconductor substrate and, more particularly, to a method for stabilizing a semiconductor substrate containing an impurity atom such as oxygen or a halogen and an epitaxial layer formed thereon.
For example, in a semiconductor device manufacturing process, a substrate which is subjected to epitaxial growth is an important material. As an example of such a substrate, a semiconductor substrate doped with boron or gallium as an impurity, and having an epitaxial layer formed thereon is known. In order to inhibit diffusion of the impurity (boron or gallium) from the epitaxial layer into the substrate, there is proposed a technique in which a metal impurity layer is formed on a substrate surface by ion implantation, and the surface is then activated using heat treatment, thereby forming an impurity layer in the substrate (e.g., 0b46394aab